single-cell whole-genome analysis Microorganisms Encapsulation AGM™ Reagent kit click here !

single-cell whole-genome analysis
Microorganisms Encapsulation AGM™ Reagent kit
click here !

3D cell culture solution | Cell Encapsulation Reagent (AGM™) | One Technologies Company3D cell culture solution | AGM™ Reagent kit | One Technologies Company

Cell Encapsulation Reagent (AGM™)

Cell Encapsulation Reagent (AGM™)

Cell Encapsulation Reagent (AGM™) | One Technologies Company

3D culture for all researchers.
3D cell culture easily achieved
in the 2D environment of a CO2 incubator.

catalog download
Cell Encapsulation AGM™ Reagent kit
click here !

Utilizes RIKEN patented technology (Patent No. 7018685)
“Agarose gel microcapsules enable easy-to-prepare, picolitre-scale, single-cell genomics, yielding high-coverage genome sequences” H Aoki, M Yuki, M Shimizu, Y Hongoh, M Ohkuma, Y Yamagata ; Scientific Reports 12, article number :17014(2022)

Cell Encapsulation Reagent (AGM™) | One Technologies Company

The latest solution for cell 3D encapsulation technology

The encapsulation technology of living cells can be used in many areas of research, including cell culture, cell transplantation, cell-based therapeutic delivery, and controlled drug delivery, because the living cells are encapsulated in a biocompatible protective capsule that maintains an optimal survival environment. Furthermore, the encapsulated membrane structure also functions as an inhibitory and protective mechanism against attacks by the autoimmune system, and is being investigated for effective use in xenotransplantation and other applications.


AGM™ Capsule Overview


The agarose capsule is a porous material of 30 to 200 nm in diameter that can quickly accommodate media exchange and gas exchange, including metabolites from cells. It also has an isolating function against foreign substances entering from outside the membrane, and provides excellent cell protection against shear damage during medium exchange, which is an issue in cell culture.

Features of AGM™

▶Cellular degeneration, which is a problem in 2D culture environments, is suppressed.
▶Cultured cells in AGM are maintained in a 3D environment similar to that in vivo.
▶The multi-closure membrane of AGMs can be used for rapid medium and gas exchange.
▶Spheroid and organoid formation can be realized in AGM.
▶The protective effect of AGM prevents shear rupture during medium exchange.
▶It can be utilized as a co-culture capsule.
Example of spheroid formation in AGM 3D culture
AGM３D培養でのスフェロイド形成例 | Cell Encapsulation Reagent (AGM™) | One Technologies Company
AGM３D培養でのスフェロイド形成例 | Cell Encapsulation Reagent (AGM™) | One Technologies Company*When cells are cultured in the AGM, they grow throughout the core and form spherical aggregates called spheroids.
The captured images show how MCF-7 and CHO-K1 cells were cultured in AGM. The porous structure of the capsule wall allows components and other materials to be fed into the capsule, and both cells were successfully cultured in the AGM. The results of culturing MCF-7 cells for 12 days (top) and the example of cells leaking from the capsule after that (bottom) are shown.The CHO-K1 cells were confirmed to be cultured without scaffold material on the 7th day.

AGM 3D Cell Culture Usage
For the acquisition and collection of extracellular metabolites such as exosomes
For separation and collection of extracellular endoplasmic reticulum, etc. utilizing AGM's porosity (φ200 nm).
For application research to Organ on Chip
Technical research utilizing spheroid/organoid and other.
Utilization as a capsule for cell preservation and transport
Can be utilized for cell preservation after culture.
Prevents shear breakdown by culture medium during transportation.
For cell-based drug efficacy evaluation research
Allows rapid chemical exchange for cultured cells.
Enables high-throughput evaluation with cells.
For food research
AGM is safe as a food additive.
As capsules for Intestinal bacteria administration.
As an immune-inhibitory membrane in xenotransplantation research
Isolation structure (agarose membrane) can suppress attacks from immune cells.
Use a droplet generator for AGM particle size uniformity
Overview of cell AGM embedding technique in manual operation
Overview of cell AGM embedding technique in manual operation | Cell Encapsulation Reagent (AGM™) | One Technologies Company

Comparison of cell embedding techniques that can clearly assess
AGM isolation characteristics

AGM
(our technology)		 Gel beads Water-in-Oil
droplet
Form AGM(our technology) | Cell Encapsulation Reagent (AGM™) | One Technologies Company Gel beads | Cell Encapsulation Reagent (AGM™) | One Technologies Company Water-in-OilWater-in-Oil droplet | Cell Encapsulation Reagent (AGM™) | One Technologies Company
Buffer, reagent exchange Easy Easy Difficult
Diffusion in capsule Easy  Difficult Easy
Unit of 1 capsule Easy Easy Difficult
Documents Aoki H, 2022 Bigdeli S, 2015 Hosokawa M, 2017
AGM
Form AGM | AGM™ Reagent kit | One Technologies Company
Buffer, reagent exchange Easy
Diffusion in capsule Easy
Unit of 1 capsule Easy
Documents Aoki H, 2022
Gel beads
Form Gel beads | AGM™ Reagent kit | One Technologies Company
Buffer, reagent exchange Easy
Diffusion in capsule Difficult
Unit of 1 capsule Easy
Documents Bigdeli S, 2015
Water-in-Oil
droplet
Form Water-in-Oil droplet | AGM™ Reagent kit | One Technologies Company
Buffer, reagent exchange Difficult
Diffusion in capsule Easy
Unit of 1 capsule Difficult
Documents Hosokawa M, 2017

｢AGM™ Reagent kit｣ Seminar

Development of AGM for animal cells

Development of AGM for animal cells Webinar | One Technologies Company

Automated Mass Encapsulation using Generator



AGM made by On-chip Droplet Generator(On-chip Biotechnologies) with the average diameter of 80 micrometer.


On-chip Droplet Generator(DG)で作製した80 µm程度のAGMの様子 | AGM™ Reagent kit | One Technologies Company

AGM™ Product Specifications

Applicable cell animal cells microorganisms
Model AGM-1000 MCM-3
(each time corresponds to 3 x 10⁶ cells)
Volume 3 times for each model
Storage Refrigerated storage required
Expiry date Within 1 year
Delivery TBD
Cell Encapsulation Reagent (AGM™) | One Technologies Company
Cell Encapsulation Reagent (AGM™)
Cell Encapsulation Reagent (AGM™) | One Technologies Company
Cell Encapsulation Reagent (AGM™)

single-cell whole-genome analysis Microorganisms Encapsulation AGM™ Reagent kit click here !

single-cell whole-genome analysis
Microorganisms Encapsulation AGM™ Reagent kit
click here !