AGM™ (Agarose Gel Microcapsules) Reagent kit

AGM™ Reagent kit | One Technologies Company

Single-cell whole-genomic analysis is a powerful tool
for studying microorganisms at the cell level.

The AGM™ reagent kit is based on the AGM (Agarose Gel Microcapsules) single-cell containment technology, which utilizes an agarose gel shell. This technology can be used in an aqueous environment for DNA amplification within the capsules, and is also useful for cell culture.

AGM™ Reagent kit | One Technologies Company

Utilizes RIKEN patented technology (Patent No. 7018685)
“Agarose gel microcapsules enable easy-to-prepare, picolitre-scale, single-cell genomics, yielding high-coverage genome sequences” H Aoki, M Yuki, M Shimizu, Y Hongoh, M Ohkuma, Y Yamagata ; Scientific Reports 12, article number :17014(2022)

AGM™ Reagent kit
introduction video

Key Benefits

Requires Small Sample Volume

Because whole genome analysis can be performed with a minimal sample size, the AGM™ reagent kit can also be used to analyze VNC (Viable but Non-Culturable) microorganisms. This ability to work with small sample volumes makes it a versatile and useful tool for a variety of applications.

Achieves High-Level of Genome Completeness

Enables the achievement of a high genome completeness rate that is not possible with traditional methods. This is particularly valuable for identifying and analyzing new and previously unknown species.

Provides Affordable and Convenient Solution

To begin single-cell whole genome analysis, you can purchase a reagent kit which allows you to perform experiments using standard physics and chemistry equipment found in most biological laboratories, without the need for costly specialized equipment. This makes it easy to get started with this type of analysis, regardless of the resources available to you.

DNA amplification using
E.coli Single Cell by MDA

Images taken by fluorescence Microscope DNA stained

DNA amplification using E.coli Single Cell by MDA | AGM™ Reagent kit | One Technologies Company

E.coli single cell contained by AGM | AGM™ Reagent kit | One Technologies Company

Left: E.coli single cell contained
by AGM
● (Green)

DNA amplified by MDA inside the AGM | AGM™ Reagent kit | One Technologies Company

Right: DNA amplified by MDA
inside the AGM
●(Green)

Comparison of Genome Completeness

Conventional Method	After the cell was isolated and placed onto a PCR plate using FACS** technology, a MDA reaction was applied.
AGM	A MDA reaction was applied to a single cell contained within AGM.

*MDA: Multiple Displacement Amplification. An isothermal DNA amplification method using Phi29 DNA polymerase and random primers in which complementary strand displacement reactions occur continuously at multiple sites.

**FACS: Fluorescence-Activated Cell Sorter. A device that can flow fluorescently labeled cells and sort specific cell populations from a heterogeneous sample into containers based on their fluorescence characteristics.

genome coverage in DNA amplification by MDA* [E. coli] | AGM™ Reagent kit | One Technologies Company

Comparison of Single Cell container method

	AGM(Toyo Method)	Gel Beads Method	Water-in-Oil Water-in-Oil Droplet
Appearance
Drug components move in and out of droplet	Yes	Yes	No
Mobility inside of the droplet	Yes	No	Yes
Taking-out of cell contained-in	Yes	Yes	No
Number of MDA reactions required*	1	2	2

	AGM
Appearance
Drug components move in and out of droplet	Yes
Mobility inside of the droplet	Yes
Taking-out of cell contained-in	Yes
Number of MDA reactions required*	1

	Gel Beads
Appearance
Drug components move in and out of droplet	Yes
Mobility inside of the droplet	No
Taking-out of cell contained-in	Yes
Number of MDA reactions required*	2

	Water-in-Oil Droplet
Appearance
Drug components move in and out of droplet	No
Mobility inside of the droplet	Yes
Taking-out of cell contained-in	No
Number of MDA reactions required*	2